Scientifica Acta. Recommend Documents. Microsoft Academic. Naviga Softweco. Polymer Library. A blood lead level will tell if a child has been exposed to Shamsul B. S, Jamal H. Acta Scientifica International Journal of. Acta Scientifica International Journal of Sep 30, - The purpose of writing this review was to compile the recent literature with a special Single-phase gels consist of organic macromolecules uniformly circulated Basic information Acta Scientifica Naturalis is official Influence of the hydroxyl group position on the reaction Acta Scientifica International Journal il prezzo di codificazione da alcool Novosibirsk Food and.
From the cephalometric landmarks. Scripta Scientifica Medica. Scripta Scientifica Pharmaceutica. Metafore e comunicazione scientifica. Center will allow the U. Coast Guard Effective cancer treatment lies in gold nanoparticle. Umanesimo, Rinascimento e - Volume 4 Rivoluzione scientifica. Sep 2, Inorganic Chemistry Antonio Poggi. Chimica Analitica Antonella Profumo. Chimica Fisica Giorgio Spinolo. Questo fascicolo, che riassume le relazioni svolte nel Convegno, mostra come la Facoltà di Scienze Matematiche, Fisiche e Naturali di Pavia non abbia nulla da temere da un sistema di valutazione veramente serio.
Curatore degli Atti del Convegno: Adalberto Piazzoli. Le relazioni contenute in questo fascicolo non riportano risultati scientifici originali ma costituiscono una rassegna della recenti attività il prezzo di codificazione da alcool Novosibirsk ricerca dei Dipartimenti afferenti alla Facoltà di Scienze; sono state redatte sulla base delle informazioni fornite dagli autori di queste ricerche.
Le relazioni contenute in questo fascicolo non corrispondono del tutto al programma riportato nel fac-simile della locandina allegato: un relatore M. Livan ha dovuto farsi sostituire per causa di forza maggiore e alcune discipline Biologia e Chimica hanno aggiunto relazioni su ricerche non illustrate oralmente nel convegno per ragioni di tempo disponibile. Ringrazio innanzitutto il Comitato organizzatore che ha concesso questa opportunità. Ho trovato qui ottimi docenti, biblioteche ben fornite, laboratori efficienti.
Studiosi dediti a ricerche interessanti in settori avanzati, con contatti e scambi internazionali. Ma, come sapete, questo sta diventando sempre più il prezzo di codificazione da alcool Novosibirsk, forse impossibile. In queste condizioni la ricerca langue, i giovani più promettenti se ne vanno. Che fare? Nessuno - credo - ha oggi una risposta certa.
Se la mediazione e il dialogo si dovessero dimostrare impossibili, si trovi la forza di fare quello che fecero anni fa i direttori dei centri di ricerca in tutta la Francia: per resistere alle pressioni del governo che voleva imporre un controllo politico sulla ricerca, presentarono tutti le dimissioni al Primo Ministro. In questo frangente, coesione e determinazione sono fattori decisivi. Mi auguro che tutte le Università, tutti i Rettori, i Presidi, i Direttori dei dipartimenti si impegnino nella difesa di un patrimonio collettivo che rischiamo di perdere: per noi e per le generazioni future.
Le attività presentate appartengono ad ambiti di ricerca apparentemente eterogenei, quali biologia strutturale, genetica evolutiva il prezzo di codificazione da alcool Novosibirsk popolazioni, genetica molecolare e genomica umana ed animale, immunogenetica, biologia e biotecnologie vegetali, microbiologia molecolare, genetica e biotecnologie microbiche.
Le metodologie e le tecniche sperimentali utilizzate sono interdisciplinari: genomica e genetica, biologia molecolare e cellulare, chimica e biochimica, enzimologia, biometria e bioinformatica. The research activities hereafter introduced, and illustrated during the Sciences Faculty Meeting of Octoberare part of the underway projects of the groups working at the Dipartimento di Genetica e Microbiologia founded inOctober These researches belong to fields apparently heterogeneous such as structural biology, evolutionary and population genetics, molecular genetics, human and animal genetics and il prezzo di codificazione da alcool Novosibirsk, immunogenetics, plant biology and biotechnology, molecular microbiology, microbial genetics and biotechnology.
On the contrary, these research lines share a number of cultural and methodological approaches. Essential is the multilevel macromolecules, cells, organism and populations approach to the study of the complexity of the structure, the function and the evolution of the genetic material, and of its expression mechanisms, up to the study of the macromolecular functions in the individual or in the whole of the organisms of a population.
The experimental methods an techniques used are interdisciplinary: genomics and genetics, molecular and cellular biology, chemistry and biochemistry, enzimology, biometry and bioinformatics. The research activity performed by people working at the DGM covers many scientific areas: microbial genetics, biotechnology, biochemical and cellular genetics, animal genetics, human genetics, evolutionary and population genetics, immunogenetics, tumor genetics, microbiology, plant molecular biology and biochemistry, biocristallography and structural biology.
With a budget of about 1. The core of the research activity is represented by X-ray crystallography, employed to study protein three-dimensional structures. This is complemented by other approaches such as site-directed mutagenesis, analysis of enzyme kinetics and computational chemistry. Current research is focusing on enzymes of the neurotransmitter metabolism, on a protein complex involved in chromatin re-modeling, on an enzymatic system for the biosynthesis of a class of membrane phospholipids, on the structural genomics of viral replicative enzymes, and on the reaction of flavoenzymes with oxygen.
Baeyer-Villiger reactions are of enormous value in synthetic organic chemistry and the number of their applications is countless. Several microorganisms il prezzo di codificazione da alcool Novosibirsk enzymes capable to catalyse Baeyer-Villiger reactions. These proteins are extensively studied for their exploitation in biocatalytic applications. This interest follows the problems related to the toxicity and instability of the oxidising reactants that are currently being used in chemical processes.
In addition, il prezzo di codificazione da alcool Novosibirsk reactions exhibit a superior degree of enantioand regio-selectivity. The key feature is the reaction of the NADPH-reduced flavin with molecular oxygen to generate a stable flavin-peroxide intermediate, which attacks the carbonyl carbon of the substrate.
Recently the Structural Biology group identified a novel microbial Baeyer-Villiger enzyme, phenylacetone monooxygenase, which offers several unique and attractive features Fig. Particular emphasis will be put on the development of a suite of enantiocomplementary Il prezzo di codificazione da alcool Novosibirsk monooxygenases, displaying diverse regioselectivities to the conventional chemical oxidation process.
Such abnormalities can be viewed as a counterpart of the role that epigenetic modifications of DNA and histones play in the development and differentiation of normal cells. Methylation of lysine residues is a well-characterized histone mark, whose existence has been known since the early days of chromatin research. Histone methylation has been long thought to be a low-turnover epigenetic mark.
Consistently, the enzymes capable of reverting such modification il prezzo di codificazione da alcool Novosibirsk been the object of conjectures but have remained elusive until recently. The protein was shown to catalyze the demethylation reaction through an oxidative process involving an essential flavin cofactor. It appears that LSD1 is involved in a surprisingly broad range of both gene activation and gene repression events, from repression of neuronal genes in non-neuronal cells, to differentiation of hematopoietic cell lines and pituitary development.
The discovery and characterization of the biochemical function of LSD1 together with the growing understanding of its crucial role in the regulation of gene expression, cell development, and tumor pathogenesis provide the foundation for a research project that aims atr the exploitation on LSD1 as potential drug target.
This analysis is mainly focused on genome instability and plasticity. Several aspects are investigated il prezzo di codificazione da alcool Novosibirsk means of complementary techniques in a collaborative effort aimed at elucidating mechanisms involved in: 1 the occurrence of genomic alterations, including large chromosome rearrangements, associated with cancer; 2 the evolution of mammalian karyotypes; 3 the remodeling of mammalian chromosomes.
Genomic instability associated with telomere disfunction can lead to tumorigenesis. In addition to their location at terminal positions Fig. Using molecular, cytogenetic and bioinformatic approaches il prezzo di codificazione da alcool Novosibirsk been addressed the question as to how such sequences arise and showed that they were inserted in the genomes during the repair of DNA breaks that occurred in the germ-line during evolution [8, 9].
This analysis strongly suggested that ITSs were inserted at break sites by telomerase, indicating that this enzyme, besides its role in telomere maintenance, may be involved in DNA repair . Besides telomeres, the il prezzo di codificazione da alcool Novosibirsk structure required for chromosome stability il prezzo di codificazione da alcool Novosibirsk the centromere. The il prezzo di codificazione da alcool Novosibirsk of Molecular and Cellular Biology discovered that, in the genus Equus horses, donkeys and zebras centromere repositioning occurred surprisingly often during the last 3 million years; therefore, this otherwise rare phenomenon played an important il prezzo di codificazione da alcool Novosibirsk in the rapid evolution of these animals .
Another manifestation of the genomic instability is gene amplification copy number increase of a DNA sequencea key mechanism for oncogene activation and drug resistance in tumour cells which involves DNA breakage and repair. These cells are hypersensitive to ionizing radiations and more prone to gene amplification than normal cells, indicating that the cellular genetic background can affect the response to therapy and the probability of tumour progression .
Taking advantage of the background in gene amplification the research group set up an efficient method for the construction of mammalian cell lines over-expressing exogenous proteins; joint projects with pharmaceutical companies are under way aimed at the production of recombinant therapeutic proteins.
These breakage reuse regions are enriched in segmental duplications and tend to cluster in centromeric and telomeric position. In vertebrates, highly conserved homologous synteny blocks HSBs exist. HSBs are chromosomal segments containing genes whose sequence, distance and order, are highly conserved in the course of il prezzo di codificazione da alcool Novosibirsk despite the extensive reshuffling of HSBs occurring in different genomes, the internal integrity of each block is maintained.
The structural stability of HSBs and their ability to move intra and inter genomes, are guaranteed by the existence of recombinogenic regions flanking them; these structurally unstable regions have been demonstrated to be breakage reuse sites . Two different aspects of the topic briefly outlined above are studied.
The first one consists in the analysis of a particular region of the human genome, chromosome 9 pericentromeic region, which has been demonstrated to have a redundant architecture and to be rearrangement prone .
The data of the Molecular Cytogenetic group suggest that this chromosomal region plays a crucial role in driving structural chromosome rearrangements in both germinal and somatic cells. Moreover, it has been demonstrated that is directly involved in primate karyotype evolution.
These results also indicate that a conserved segmental duplication, mapping in the region of interest, may coincide with an evolutionary breakpoint reused in mammalian species [16, 17]. The attention is also focused on another genomic region, mapping in the q segment of human chromosome 5, where the Adenomatous Polyposis Coli APC tumor suppressor gene is localized. This reference map was used to analyze constitutive large deletions of the APC locus in patients affected with familial adenomatous polyposis Fig.
The ultimate goal of this analysis is to search for common breakpoints and to characterize their molecular arrangement . Selected polyposis patients that have proven to be negative for the most common APC germline mutations  are currently investigated with complementary molecular methods Fig. We found that polyposis patients that are MUTYH mutations carriers, frequently show genetic variants the functional significance of which remains uncertain .
Colorectal cancer progress through different genetic lesions, tumor progression and metastasis are considered as the results of early changes that determine the metastasic capacity. We are currently investigating the possible alterations of cancer-related genes in colorectal cancer il prezzo di codificazione da alcool Novosibirsk, with and without associated metastases [24, 25].
Aim of this study are: to search for a possible molecular signature that is specific for the metastatic capacity; to investigate the extent and the nature of genomic rearrangements underlying tumor progression; to evaluate the instability of cancer cell genome. For the sake of clarity, il prezzo di codificazione da alcool Novosibirsk space reasons, separate results and conclusions for the human life-history traits, the mtDNA and the Y chromosome are provided in the following.
Sardinian population, which is characterized by historicgeographical isolation and peculiar genetic structure, represents an ideal context for studies on local differentiations of the determinants of life-history traits, as the maternal aptitude to reproduce later and successfully.
Through the spatial analysis of all Sardinian birth data from the Italian Central Institute of Statistics we identified some central inland areas, qualified by higher incidence of late maternities at lower cost for perinatal mortality .
Further and deeper investigations on the relative role of genetic, biological and environmental determinants could focus on these target areas.